HBcAg (1H8) Monoclonal Antibody

$Antigen: bs-2000P, 0.2ug\/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat Anti-Mouse IgG (bs-0296G-HRP) at 1: 5000; TMB staining; Read the data in MicroplateReader by 450nm.$

Antigen: bs-2000P, 0.2ug\/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat Anti-Mouse IgG (bs-0296G-HRP) at 1: 5000; TMB staining; Read the data in MicroplateReader by 450nm.

$Lane 1: Recombinant HBcAg protein, Tag free probed with HBcAg Monoclonal Antibody, Unconjugated (bsm-2000M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant HBcAg protein, Tag free probed with HBcAg Monoclonal Antibody, Unconjugated (bsm-2000M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IF(IHC-P)

Reactivity

Virus
Others

Overview
Catalog #	bsm-2000m-100ul
Product Name	HBcAg (1H8) Monoclonal Antibody
Applications	ELISA, IHC-P, IF(IHC-P)
Reactivity	Virus, Others
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant Hepatitis B Core Antigen (rHBcAg)
Clonality	Monoclonal
Clone #	1H8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M PBS (pH7.4) with 0.02% Proclin300.
Storage Condition	Store at -20°C for 12 months.
Target
Synonyms	C; Capsid protein; Core and e antigen; Core antigen; Core antigen; Core protein; HBc; HBcAg; HBVgp4; Hepatitis B Virus core antigen; p21.5; precore/core protein; CAPSD_HBVA3.
Background	Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection.The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200