OPG Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-62482r
Product Name	OPG Recombinant Antibody
Applications	IHC-P IHC-F IF(IHC-P) IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human TNFRSF11B
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4982
Swiss Prot	O00300
Subcellular location	Secreted
Synonyms	OCIF; OPG; PDB5; TR1; TR11B_HUMAN; TNFRSF11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNF receptor superfamily member 11b; tumor necrosis factor receptor superfamily, member 11b
Background	Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B.
Application Dilution
IHC-P	1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500
IF(ICC)	IFICC1:50-200