HuR / ELAVL1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Lane 1: Hela cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: 293T cell lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Thymus lysates probed with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Hela cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: 293T cell lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Thymus lysates probed with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: 293T cell lysates; Lane 2: HuR \/ ELAVL1 knockout (KO) 293T cell lysates probed with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293T cell lysates; Lane 2: HuR \/ ELAVL1 knockout (KO) 293T cell lysates probed with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HuR \/ ELAVL1) polyclonal Antibody, Unconjugated (bs-55106R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HuR \/ ELAVL1) polyclonal Antibody, Unconjugated (bs-55106R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IHC
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55106R
Product Name	HuR / ELAVL1 Polyclonal Antibody
Applications	WB, IHC-P, IHC, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	1994
Swiss Prot	Q15717
Subcellular location	Cytoplasm, Nucleus
Synonyms	ELAV1;HUR;Hua;MelG;HuR / ELAVL1;ELAVL1
Background	The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC
IF