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Recombinant Antibodies

Recombinant Antibodies


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Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RAF1 (Ser43) Monoclonal Antibody, Unconjugated (bsm-52195R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RAF1 (Ser43) Monoclonal Antibody, Unconjugated (bsm-52195R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RAF1 (Ser43) Monoclonal Antibody, Unconjugated (bsm-52195R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RAF1 (Ser43) Monoclonal Antibody, Unconjugated (bsm-52195R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52195r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 1 Monoclonal Antibody, Unconjugated(bsm-52051R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 1 Monoclonal Antibody, Unconjugated(bsm-52051R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 1 Monoclonal Antibody, Unconjugated(bsm-52051R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Cytokeratin 1 monoclonal antibody, unconjugated (bsm-52051R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52051r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52024r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PPAR gamma monoclonal antibody, unconjugated (bsm-52220R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-PPAR gamma antibody (bs-52220R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PPAR gamma) monoclonal Antibody, unconjugated (bsm-52220R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-52220r-TR

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TrkB(Y817) (2A1) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates probed with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52213r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Raji cell lysates probed with Catalase Monoclonal Antibody, Unconjugated (bsm-60450M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60450m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v7801-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v7803-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v7802-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v5811-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v5810-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v5808-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v5604-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v22803-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v20104-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: v20105-1mg

Applications: ELISA, LFA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52375r-cy5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-52375r-hrp

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-52375r-rbitc

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-52375r-cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-52375r-pe

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-52375r-percp-cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52375r-pe-cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-52375r-pe-cy5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-52375r-cy3

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-52375r-percp

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-52375r-bf680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52375r-pe-cy7

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-52375r-fitc

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-52375r-cy7

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-52375r-biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-52375r-bf750

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-52375r-bf647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52375r-bf594

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52375r-bf555

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-52375r-apc-cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-52375r-bf488

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-52375r-bf405

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-52375r-apc-cy7

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-52375r-bf350

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-52375r-apc

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-34317r-pe-cy7

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-34317r-rbitc

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-34317r-hrp

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-34317r-bf594

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-34317r-percp

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-34317r-cy5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-34317r-cy5.5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5