Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

NF-L Recombinant Antibody

The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-NF-L antibody (bsm-41836R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-NF-L antibody (bsm-41836R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (bsm-41836R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (bsm-41836R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (bsm-41836R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (bsm-41836R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-41836r-100uL
Product Name NF-L Recombinant Antibody
Applications
FLOW-CYT ICC/IF
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human NF-L protein
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4747
Swiss Prot P07196
Subcellular location Cytoplasm
Synonyms CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110; NFL_HUMAN; NEFL; 68 kDa neurofilament protein; Neurofilament triplet L protein; NFL_MOUSE; NFL_RAT;
Application Dilution
Flow-Cyt =1μg/Test
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is NF-L Recombinant Antibody (bsm-41836R) validated for, and at what dilution?

bsm-41836R is validated for Flow-Cyt (Flow-Cyt=1μg/Test); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NF-L Recombinant Antibody (bsm-41836R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-41836R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NF-L Recombinant Antibody (bsm-41836R)?

Recombinant human NF-L protein

Search our catalog of hundreds of thousands of products

Free sample offer

Try a recombinant sample

20 µL sample

Freewith your paid order

View product details (opens in a new tab)

View cart

US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

Primary antibody added to your cart

Need a secondary antibody?

Product validation image

View product details and validation

Confirm application and cross-reactivity before use. Prices shown are before eligible cart discounts. Shipping and tax are calculated at checkout.

View cart