Aurora B Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52019r-20uL |
| Product Name | Aurora B Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant human Aurora B protein, around N-terminal 150aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 9212 |
| Swiss Prot | Q96GD4 |
| Synonyms | Aurora B phospho Y12; Aurora B phospho T12; AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5. |
| Background | Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is Aurora B Recombinant Antibody (bsm-52019R) validated for, and at what dilution?
bsm-52019R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Aurora B Recombinant Antibody (bsm-52019R) react with?
Tested reactivity: Human.
How should bsm-52019R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Aurora B Recombinant Antibody (bsm-52019R)?
Recombinant human Aurora B protein, around N-terminal 150aa.