Cyclin E2 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52049r-rbitc-100uL |
| Product Name | Cyclin E2 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Recombinant human Cyclin E2 protein, around C-terminal 100aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9134 |
| Swiss Prot | O96020 |
| Subcellular location | Nucleus |
| Synonyms | Cyclin E2 phospho T392; p-Cyclin E2 phospho T392;CCN E2; CCNE 2; CCNE2; CCNE2 protein; CYC E2; CYCE 2; CYCE2; CyclinE2; G1/S-specic cyclin E2; G1/S-specic cyclin E2. |
| Background | The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Cyclin E2 Recombinant Antibody, RBITC Conjugated (bsm-52049R-RBITC) validated for, and at what dilution?
bsm-52049R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cyclin E2 Recombinant Antibody, RBITC Conjugated (bsm-52049R-RBITC) react with?
Tested reactivity: Human.
How should bsm-52049R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cyclin E2 Recombinant Antibody, RBITC Conjugated (bsm-52049R-RBITC)?
Recombinant human Cyclin E2 protein, around C-terminal 100aa.
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-52049R-RBITC)?
RBITC on bsm-52049R-RBITC: excitation / emission 570nm/595nm.