CK10/Cytokeratin 10 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52052r-bf647-100uL |
| Product Name | CK10/Cytokeratin 10 Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | Recombinant human Cytokeratin 10 protein, around 150-250aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3858 |
| Swiss Prot | P13645 |
| Synonyms | BIE; EHK; K10; KPP; BCIE; CK10; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; KRT10 |
| Background | Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is CK10/Cytokeratin 10 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52052R-BF647) validated for, and at what dilution?
bsm-52052R-BF647 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CK10/Cytokeratin 10 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52052R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52052R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CK10/Cytokeratin 10 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52052R-BF647)?
Recombinant human Cytokeratin 10 protein, around 150-250aa.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52052R-BF647)?
AbBy Fluor-647 on bsm-52052R-BF647: excitation / emission 650nm/665nm.