phospho-EGFR (Ser695) Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52148r-apc-100uL |
| Product Name | phospho-EGFR (Ser695) Recombinant Antibody, APC Conjugated |
| Applications |
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | Synthetic peptide derived from human EGFR(S695), around 680-710aa (phospho S695). |
| Modification Site | S695 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1956 |
| Swiss Prot | P00533 |
| Synonyms | ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR |
| Background | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action. |
| Application Dilution | |
| IF(IHC-P) | 1:100-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is phospho-EGFR (Ser695) Recombinant Antibody, APC Conjugated (bsm-52148R-APC) validated for, and at what dilution?
bsm-52148R-APC is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-EGFR (Ser695) Recombinant Antibody, APC Conjugated (bsm-52148R-APC) react with?
Tested reactivity: Human.
How should bsm-52148R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-EGFR (Ser695) Recombinant Antibody, APC Conjugated (bsm-52148R-APC)?
Synthetic peptide derived from human EGFR(S695), around 680-710aa (phospho S695).
What are the excitation and emission wavelengths of the APC conjugate (bsm-52148R-APC)?
APC on bsm-52148R-APC: excitation / emission 650nm/660nm.