eIF4E (Ser209) Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52152r-pe-100uL |
| Product Name | eIF4E (Ser209) Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 |
| Modification Site | S209 |
| Clonality | Recombinant |
| Clone # | 2B1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1977 |
| Swiss Prot | P06730 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | AUTS19; CBP; EIF4E1; EIF4EL1; EIF4F; eIF-4E; EG668879; Eif4e-ps; If4e; IF4E_HUMAN; EIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; IF4E_MOUSE; IF4E_RAT; eukaryotic translation initiation factor 4E; eIF4E (phospho-S209); p-eIF4E; phospho-eIF4E; eIF4E (phospho-Ser209) |
| Background | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is eIF4E (Ser209) Recombinant Antibody, PE Conjugated (bsm-52152R-PE) validated for, and at what dilution?
bsm-52152R-PE is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4E (Ser209) Recombinant Antibody, PE Conjugated (bsm-52152R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52152R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4E (Ser209) Recombinant Antibody, PE Conjugated (bsm-52152R-PE)?
KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209
What are the excitation and emission wavelengths of the PE conjugate (bsm-52152R-PE)?
PE on bsm-52152R-PE: excitation / emission 496,564nm/578nm.