Phospho-Estrogen Receptor alpha (Ser118) Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52154r-fitc-100uL |
| Product Name | Phospho-Estrogen Receptor alpha (Ser118) Recombinant Antibody, FITC Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | Synthetic peptide derived from human Estrogen Receptor alpha(S118), around 180-220aa (phospho S209). |
| Modification Site | S118 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2099 |
| Swiss Prot | P03372 |
| Synonyms | ER; ESR; Era; ESRA; ESTRR; NR3A1; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1 |
| Background | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is Phospho-Estrogen Receptor alpha (Ser118) Recombinant Antibody, FITC Conjugated (bsm-52154R-FITC) validated for, and at what dilution?
bsm-52154R-FITC is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Estrogen Receptor alpha (Ser118) Recombinant Antibody, FITC Conjugated (bsm-52154R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52154R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Estrogen Receptor alpha (Ser118) Recombinant Antibody, FITC Conjugated (bsm-52154R-FITC)?
Synthetic peptide derived from human Estrogen Receptor alpha(S118), around 180-220aa (phospho S209).
What are the excitation and emission wavelengths of the FITC conjugate (bsm-52154R-FITC)?
FITC on bsm-52154R-FITC: excitation / emission 494nm/518nm.