Rb (Ser807) Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52197r-bf350-100uL |
| Product Name | Rb (Ser807) Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Ser807 |
| Modification Site | S807 |
| Clonality | Recombinant |
| Clone # | 2D10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5925 |
| Swiss Prot | P06400 |
| Subcellular location | Nucleus |
| Synonyms | OSRC; PPP1R130; RB; p105-Rb; p110-RB1; pRb; pp110; Rb-1; pp105; RB_HUMAN; RB1; pRb (Rb); RB_MOUSE; RB_RAT; RB transcriptional corepressor 1; osteosarcoma; retinoblastoma 1; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; RB1 | Rb (phospho-S807); p-Rb; phospho-Rb; p-RB1; RB1 | Rb (phospho-Ser807) |
| Background | Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is Rb (Ser807) Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52197R-BF350) validated for, and at what dilution?
bsm-52197R-BF350 is validated for WB (1:500-2000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rb (Ser807) Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52197R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52197R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Rb (Ser807) Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52197R-BF350)?
KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Ser807
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-52197R-BF350)?
AbBy Fluor-350 on bsm-52197R-BF350: excitation / emission 346nm/442nm.