SIRT1 (Thr530) Recombinant Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52202r-bf750-100uL |
| Product Name | SIRT1 (Thr530) Recombinant Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human SIRT1 around the phosphorylation site of Thr530 |
| Modification Site | T530 |
| Clonality | Recombinant |
| Clone # | 6H5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23411 |
| Swiss Prot | Q96EB6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | SIR2; SIR2L1; SIR2alpha; Sir2a; SIR1_HUMAN; SIRT1; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); 2.3.1.286; SIR1_MOUSE; SIR2-like protein 1; SIR2alpha (Sir2 | mSIR2a); sirtuin 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); SIRT1 (phospho-T530); p-SIRT1; phospho-SIRT1; SIRT1 (phospho-Thr530) |
| Background | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] |
| Application Dilution | |
| WB | 1:500-1000 |
Frequently asked questions
Which applications is SIRT1 (Thr530) Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-52202R-BF750) validated for, and at what dilution?
bsm-52202R-BF750 is validated for WB (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SIRT1 (Thr530) Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-52202R-BF750) react with?
Tested reactivity: Human.
How should bsm-52202R-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SIRT1 (Thr530) Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-52202R-BF750)?
KLH conjugated synthesised phosphopeptide derived from human SIRT1 around the phosphorylation site of Thr530
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-52202R-BF750)?
AbBy Fluor-750 on bsm-52202R-BF750: excitation / emission 749nm/775nm.