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NeuN Recombinant Antibody

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates probed with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates probed with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NeuN antibody (bsm-52268R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NeuN antibody (bsm-52268R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\n Merged staining of anti-NF-H (bsm-52851R; 1:200; orange), anti-NeuN (bsm-52268R; 1:200; green).DAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\n Merged staining of anti-NF-H (bsm-52851R; 1:200; orange), anti-NeuN (bsm-52268R; 1:200; green).DAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\n Merged staining of anti-NF-H (bsm-52851R; 1:200; orange),anti-GFAP (bsm-42001R; 1:200; green), anti-NeuN (bsm-52268R; 1:200; pink).DAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\n Merged staining of anti-NF-H (bsm-52851R; 1:200; orange),anti-GFAP (bsm-42001R; 1:200; green), anti-NeuN (bsm-52268R; 1:200; pink).DAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\n Merged staining of anti-NF-H (bsm-52851R; 1:200; orange),anti-GFAP (bsm-42001R; 1:200; green), anti-NeuN (bsm-52268R; 1:200; pink).DAPI (dark blue) was used as a nuclear counter stain.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\n Merged staining of anti-NF-H (bsm-52851R; 1:200; orange),anti-GFAP (bsm-42001R; 1:200; green), anti-NeuN (bsm-52268R; 1:200; pink).DAPI (dark blue) was used as a nuclear counter stain.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52268r-20uL
Product Name NeuN Recombinant Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Human NeuN between 1-100 amino acids
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 146713
Swiss Prot A6NFN3
Synonyms RNA binding protein fox-1 homolog 3, Fox-1 homolog C, RBFOX3
Background Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF

Frequently asked questions

Which applications is NeuN Recombinant Antibody (bsm-52268R) validated for, and at what dilution?

bsm-52268R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NeuN Recombinant Antibody (bsm-52268R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52268R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise NeuN Recombinant Antibody (bsm-52268R)?

Human NeuN between 1-100 amino acids

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