NADPH oxidase 4 Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52390r-biotin-100uL |
| Product Name | NADPH oxidase 4 Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-F
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Recombinant human NADPH oxidase 4 between 500 to C-terminus |
| Clonality | Monoclonal |
| Clone # | 2A4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 50507 |
| Swiss Prot | Q9NPH5 |
| Synonyms | NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, KOX-1, NOX4, RENOX |
| Background | Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | IHC-F1:50-200 |
Frequently asked questions
Which applications is NADPH oxidase 4 Recombinant Antibody, Biotin Conjugated (bsm-52390R-Biotin) validated for, and at what dilution?
bsm-52390R-Biotin is validated for WB(WB) (1:500-2000); IHC-F(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NADPH oxidase 4 Recombinant Antibody, Biotin Conjugated (bsm-52390R-Biotin) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-52390R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NADPH oxidase 4 Recombinant Antibody, Biotin Conjugated (bsm-52390R-Biotin)?
Recombinant human NADPH oxidase 4 between 500 to C-terminus