ICAM1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52466r-rbitc-100uL |
| Product Name | ICAM1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | A synthesized peptide derived from human ICAM 1 |
| Clonality | Recombinant |
| Clone # | 1G2 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3383 |
| Swiss Prot | P05362 |
| Subcellular location | Cell membrane |
| Synonyms | BB2; CD54; P3.58; Icam-1; Ly-47; MALA-2; ICAM; ICAM1_HUMAN; ICAM1; Major group rhinovirus receptor; ICAM1_MOUSE; MyD10; ICAM1_RAT; intercellular adhesion molecule 1; human rhinovirus receptor |
| Background | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF | 1:200-1000 |
Frequently asked questions
Which applications is ICAM1 Recombinant Antibody, RBITC Conjugated (bsm-52466R-RBITC) validated for, and at what dilution?
bsm-52466R-RBITC is validated for WB (1:1000-5000); IF (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ICAM1 Recombinant Antibody, RBITC Conjugated (bsm-52466R-RBITC) react with?
Tested reactivity: Human.
How should bsm-52466R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ICAM1 Recombinant Antibody, RBITC Conjugated (bsm-52466R-RBITC)?
A synthesized peptide derived from human ICAM 1
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-52466R-RBITC)?
RBITC on bsm-52466R-RBITC: excitation / emission 570nm/595nm.