Cyclooxygenase 2 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52502r-bf680-100uL |
| Product Name | Cyclooxygenase 2 Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human COX2 |
| Clonality | Recombinant |
| Clone # | 14D5 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5743 |
| Swiss Prot | P35354 |
| Subcellular location | Cytoplasm |
| Synonyms | COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; PES-2; PHS II; Pghs2; TIS10; PGH2_HUMAN; PTGS2; Cyclooxygenase-2 (COX-2); Prostaglandin H2 synthase 2 (PGH synthase 2 | PGHS-2); Prostaglandin-endoperoxide synthase 2; 1.14.99.1; PGH2_MOUSE; Glucocorticoid-regulated inflammatory cyclooxygenase; Macrophage activation-associated marker protein P71/73; TIS10 protein; Pghs-b; PGH2_RAT; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); prostaglandin G/H synthase 2; cyclooxygenase 2 |
| Background | Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF | 1:200-500 |
Frequently asked questions
Which applications is Cyclooxygenase 2 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52502R-BF680) validated for, and at what dilution?
bsm-52502R-BF680 is validated for WB (1:1000-2000); IF (1:200-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cyclooxygenase 2 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52502R-BF680) react with?
Tested reactivity: Human, Mouse.
How should bsm-52502R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cyclooxygenase 2 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52502R-BF680)?
A synthesized peptide derived from human COX2
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-52502R-BF680)?
AbBy Fluor-680 on bsm-52502R-BF680: excitation / emission 679nm/702nm.