HLA-DR Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52535r-bf405-100uL |
| Product Name | HLA-DR Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | Recombinant human HLA-DR between 150-250 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3122 |
| Swiss Prot | P01903 |
| Synonyms | HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA |
| Background | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF(IHC-P) | IFIHC-P1:500-2000 |
Frequently asked questions
Which applications is HLA-DR Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52535R-BF405) validated for, and at what dilution?
bsm-52535R-BF405 is validated for WB(WB) (1:1000-5000); IF(IHC-P)(IF(IHC-P)) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HLA-DR Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52535R-BF405) react with?
Tested reactivity: Human.
How should bsm-52535R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HLA-DR Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-52535R-BF405)?
Recombinant human HLA-DR between 150-250 amino acids
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-52535R-BF405)?
AbBy Fluor-405 on bsm-52535R-BF405: excitation / emission 401nm/421nm.