CD3D Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52744r-bf647-100uL |
| Product Name | CD3D Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human CD3 delta |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 915 |
| Swiss Prot | P04234 |
| Subcellular location | Cell membrane |
| Synonyms | CD3-DELTA; CD3DELTA; IMD19; T3D; CD3D_HUMAN; CD3D; T-cell receptor T3 delta chain; CD3D_MOUSE; CD3 delta subunit of T-cell receptor complex; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3d molecule; T-cell surface glycoprotein CD3 delta chain |
| Background | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456). |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF | 1:500-2000 |
Frequently asked questions
Which applications is CD3D Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52744R-BF647) validated for, and at what dilution?
bsm-52744R-BF647 is validated for WB (1:1000-5000); IF (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD3D Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52744R-BF647) react with?
Tested reactivity: Human.
How should bsm-52744R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD3D Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52744R-BF647)?
A synthesized peptide derived from human CD3 delta
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52744R-BF647)?
AbBy Fluor-647 on bsm-52744R-BF647: excitation / emission 650nm/665nm.