TFRC Recombinant Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52793r-pe-100uL |
| Product Name | TFRC Recombinant Antibody, PE Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | Human Transferrin Receptor between 100 to 450 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | P02786 |
| Swiss Prot | P02786 |
| Synonyms | Transferrin receptor protein 1, T9, p90, CD_antigen: CD71, TR, TfR, TfR1, Trfr, TFRC |
| Background | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is TFRC Recombinant Antibody, PE Conjugated (bsm-52793R-PE) validated for, and at what dilution?
bsm-52793R-PE is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TFRC Recombinant Antibody, PE Conjugated (bsm-52793R-PE) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-52793R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TFRC Recombinant Antibody, PE Conjugated (bsm-52793R-PE)?
Human Transferrin Receptor between 100 to 450 amino acids
What are the excitation and emission wavelengths of the PE conjugate (bsm-52793R-PE)?
PE on bsm-52793R-PE: excitation / emission 496,564nm/578nm.