AHR Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52886r-100uL |
| Product Name | AHR Recombinant Antibody |
| Applications |
WB
IHC-P
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human Aryl hydrocarbon Receptor aa 600-800 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 196 |
| Swiss Prot | P35869 |
| Synonyms | Class E basic helix-loop-helix protein 76, Ah receptor, AhR, bHLHe76 |
| Background | Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates (PubMed:10395741, PubMed:28602820, PubMed:7961644, PubMed:23275542, PubMed:30373764). Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed:7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed:7961644). Involved in cell-cycle regulation (PubMed:12213388). Likely to play an important role in the development and maturation of many tissues (PubMed:12213388). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed:28602820). Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AHR Recombinant Antibody (bsm-52886R) validated for, and at what dilution?
bsm-52886R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AHR Recombinant Antibody (bsm-52886R) react with?
Tested reactivity: Human, Mouse.
How should bsm-52886R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise AHR Recombinant Antibody (bsm-52886R)?
Human Aryl hydrocarbon Receptor aa 600-800