PDPK1 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-54037r-bf488-100uL |
| Product Name | PDPK1 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDK1 |
| Clonality | Recombinant |
| Clone # | 2F9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5170 |
| Swiss Prot | O15530 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PDK1; PDPK2; PDPK2P; PRO0461; PDPK1_HUMAN; PDPK1; hPDK1; 2.7.11.1; PDPK1_MOUSE; mPDK1; PDPK1_RAT; Protein kinase B kinase (PkB kinase); 3-phosphoinositide dependent protein kinase 1; PkB kinase |
| Background | Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2). |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is PDPK1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54037R-BF488) validated for, and at what dilution?
bsm-54037R-BF488 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDPK1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54037R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-54037R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDPK1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54037R-BF488)?
KLH conjugated synthetic peptide derived from human PDK1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-54037R-BF488)?
AbBy Fluor-488 on bsm-54037R-BF488: excitation / emission 499nm/519nm.