MUC-4 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54070R-100uL |
| Product Name | MUC-4 Recombinant Antibody |
| Applications |
WB
IHC-P
FCM
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | C terminal Human MUC4 |
| Clonality | Monoclonal |
| Clone # | 3A1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 4585 |
| Swiss Prot | Q99102 |
| Synonyms | Mucin-4, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin-4 alpha chain, Ascites sialoglycoprotein 1, Mucin-4 beta chain, Ascites sialoglycoprotein 2, MUC-4, ASGP, ASGP-1, ASGP-2, MUC4 |
| Background | May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is MUC-4 Recombinant Antibody (bsm-54070R) validated for, and at what dilution?
bsm-54070R is validated for WB (1:300-5000); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MUC-4 Recombinant Antibody (bsm-54070R) react with?
Tested reactivity: Human, Rat.
How should bsm-54070R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MUC-4 Recombinant Antibody (bsm-54070R)?
C terminal Human MUC4