Growth hormone receptor Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-54199R-100uL |
| Product Name | Growth hormone receptor Recombinant Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide within human Growth hormone receptor aa 500-630 |
| Clonality | Monoclonal |
| Clone # | 3C5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2690 |
| Swiss Prot | P10912 |
| Synonyms | Growth hormone receptor; Somatotropin receptor; Growth hormone-binding protein; Serum-binding protein; GH receptor; GH-binding protein; GHBP; GHR. |
| Background | GHR is a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. It is a receptor for pituitary gland growth hormone involved in regulating postnatal body growth. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:50-200 |
| FCM | 10ul/10^6 cells in 100ul |
Frequently asked questions
Which applications is Growth hormone receptor Recombinant Antibody (bsm-54199R) validated for, and at what dilution?
bsm-54199R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:50-200); FCM (10ul/10^6 cells in 100ul). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Growth hormone receptor Recombinant Antibody (bsm-54199R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-54199R be stored?
Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Growth hormone receptor Recombinant Antibody (bsm-54199R)?
Synthetic peptide within human Growth hormone receptor aa 500-630