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Nuclear Matrix Protein p84 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Nuclear Matrix Protein p84 monoclonal antibody, unconjugated (bsm-54202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Nuclear Matrix Protein p84 monoclonal antibody, unconjugated (bsm-54202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54202r-100uL
Product Name Nuclear Matrix Protein p84 Recombinant Antibody
Applications
WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nuclear Matrix Protein p84
Clonality Recombinant
Clone # 6C1
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 9984
Swiss Prot Q96FV9
Subcellular location Cytoplasm, Nucleus
Synonyms DFNA86; HPR1; P84; P84N5; 3110002N20Rik; NMP-84; tho1; LRRGT00070; THOC1_HUMAN; THOC1; Nuclear matrix protein p84 (p84N5); hTREX84; THOC1_MOUSE; Nuclear matrix protein p84; THOC1_RAT; Liver regeneration-related protein LRRG175; THO complex subunit 1; THO complex 1
Background THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy.
Application Dilution
WB 1:500-1000

Frequently asked questions

Which applications is Nuclear Matrix Protein p84 Recombinant Antibody (bsm-54202R) validated for, and at what dilution?

bsm-54202R is validated for WB(WB) (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nuclear Matrix Protein p84 Recombinant Antibody (bsm-54202R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-54202R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.

What immunogen was used to raise Nuclear Matrix Protein p84 Recombinant Antibody (bsm-54202R)?

KLH conjugated synthetic peptide derived from human Nuclear Matrix Protein p84

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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