Versican Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54256R-100uL |
| Product Name | Versican Recombinant Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Versican aa 3100-3300 |
| Clonality | Monoclonal |
| Clone # | 2G2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 1462 |
| Swiss Prot | P13611 |
| Subcellular location | Extracellular, Secreted |
| Synonyms | Versican core protein; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; Chondroitin sulfate proteoglycan 2; GHAP; VCAN; CSPG2. |
| Background | Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a lectin-like sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycosaminoglycan chains. Accumulation around smooth muscle cells in lesions of atherosclerosis, suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may facilitate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels. The human versican gene maps to chromosome 5q14.3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Versican Recombinant Antibody (bsm-54256R) validated for, and at what dilution?
bsm-54256R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Versican Recombinant Antibody (bsm-54256R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54256R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Versican Recombinant Antibody (bsm-54256R)?
Recombinant protein within human Versican aa 3100-3300