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TGF alpha Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF alpha Monoclonal Antibody, Unconjugated (bsm-54286R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IP
  • IF
  • IHC-P
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-54286R-100uL
Product Name TGF alpha Recombinant Antibody
Applications
WB IHC-F IP IF IHC-P ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant full length protein of human TGF alpha
Clonality Monoclonal
Clone # 1H8
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7039
Swiss Prot P01135
Subcellular location Extracellular, Secreted, Cell membrane
Synonyms Protransforming growth factor alpha; Transforming growth factor alpha; EGF-like TGF; TGF type 1; TGF-alpha; ETGF; TGFA
Background TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Application Dilution
WB 1:300-5000
IHC-F 1:50-200
IP 1-2ug
IF 1:50-200
IHC-P 1:50-200
ICC/IF 1:50-200

Frequently asked questions

Which applications is TGF alpha Recombinant Antibody (bsm-54286R) validated for, and at what dilution?

bsm-54286R is validated for WB (1:300-5000); IHC-F (1:50-200); IP (1-2ug); IF (1:50-200); IHC-P (1:50-200); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TGF alpha Recombinant Antibody (bsm-54286R) react with?

Tested reactivity: Human.

How should bsm-54286R be stored?

Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TGF alpha Recombinant Antibody (bsm-54286R)?

Recombinant full length protein of human TGF alpha

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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