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Desmoglein 2 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Desmoglein 2 Monoclonal Antibody, Unconjugated(bsm-54310R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Desmoglein 2 Monoclonal Antibody, Unconjugated(bsm-54310R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Desmoglein 2) monoclonal Antibody, unconjugated (bsm-54310R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Desmoglein 2) monoclonal Antibody, unconjugated (bsm-54310R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Desmoglein 2 antibody (bsm-54310R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Desmoglein 2 antibody (bsm-54310R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with Desmoglein 2 monoclonal antibody, unconjugated (bsm-54310R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Desmoglein 2 monoclonal antibody, unconjugated (bsm-54310R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-54310r-100uL
Product Name Desmoglein 2 Recombinant Antibody
Applications
WB FLOW-CYT ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within C-terminal human Desmoglein 2
Clonality Recombinant
Clone # 7D6
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1829
Swiss Prot Q14126
Subcellular location Cell membrane
Synonyms CDHF5; HDGC; D18Ertd293e; DSG2_HUMAN; DSG2; Cadherin family member 5; DSG2_MOUSE; desmoglein 2
Application Dilution
WB 1:500-2000
Flow-Cyt 1:50-100
ICC/IF 1:50-200

Frequently asked questions

Which applications is Desmoglein 2 Recombinant Antibody (bsm-54310R) validated for, and at what dilution?

bsm-54310R is validated for WB (1:500-2000); Flow-Cyt (1:50-100); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Desmoglein 2 Recombinant Antibody (bsm-54310R) react with?

Tested reactivity: Human.

How should bsm-54310R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Desmoglein 2 Recombinant Antibody (bsm-54310R)?

Recombinant protein within C-terminal human Desmoglein 2

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