Bub3 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54413r-cy3-100uL |
| Product Name | Bub3 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant human Bub3 |
| Clonality | Recombinant |
| Clone # | 24D1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9184 |
| Swiss Prot | O43684 |
| Subcellular location | Nucleus |
| Synonyms | BUB3L; hBUB3; CR201_G0012679; Aa2-050; BUB3_BOVIN; BUB3; BUB3_HUMAN; BUB3_MOUSE; WD repeat type I transmembrane protein A72.5; BUB3_PONAB; BUB3 mitotic checkpoint protein; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; budding uninhibited by benzimidazoles 3 homolog (yeast) |
| Background | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is Bub3 Recombinant Antibody, Cy3 Conjugated (bsm-54413R-Cy3) validated for, and at what dilution?
bsm-54413R-Cy3 is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bub3 Recombinant Antibody, Cy3 Conjugated (bsm-54413R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54413R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bub3 Recombinant Antibody, Cy3 Conjugated (bsm-54413R-Cy3)?
Recombinant human Bub3
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54413R-Cy3)?
Cy3 on bsm-54413R-Cy3: excitation / emission 512,550nm/570,615nm.