BRD2 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54434r-rbitc-100uL |
| Product Name | BRD2 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Recombinant protein within human BRD2 aa 1-200. |
| Immunogen Range | 1-50/801 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6046 |
| Swiss Prot | P25440 |
| Subcellular location | Nucleus |
| Synonyms | Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein; BRD2; KIAA9001; RING3 |
| Background | The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The bromodomain-containing proteins are ubiquitously expressed. BRD2 may play a role in spermatogenesis or folliculogenesis (By similarity). BRD2 binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is BRD2 Recombinant Antibody, RBITC Conjugated (bsm-54434R-RBITC) validated for, and at what dilution?
bsm-54434R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BRD2 Recombinant Antibody, RBITC Conjugated (bsm-54434R-RBITC) react with?
Tested reactivity: Human.
How should bsm-54434R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BRD2 Recombinant Antibody, RBITC Conjugated (bsm-54434R-RBITC)?
Recombinant protein within human BRD2 aa 1-200. Immunogen range: 1-50/801.
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-54434R-RBITC)?
RBITC on bsm-54434R-RBITC: excitation / emission 570nm/595nm.