BANF1 Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54435r-fitc-100uL |
| Product Name | BANF1 Recombinant Antibody, FITC Conjugated |
| Applications |
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | Recombinant human BANF1 |
| Clonality | Recombinant |
| Clone # | 11H3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8815 |
| Swiss Prot | O75531 |
| Subcellular location | Nucleus, Cell membrane |
| Synonyms | BAF; BCRP1; D14S1460; NGPS; L2bp1/Baf; L2bp1; BAF_HUMAN; BANF1; Breakpoint cluster region protein 1; BCRG1; BAF_MOUSE; LAP2-binding protein 1; BAF_RAT; barrier to autointegration nuclear assembly factor 1; BAF nuclear assembly factor 1; barrier to autointegration factor 1 |
| Background | Barrier-to-autointegration factor (BANF1) binds non-specifically to double stranded DNA, possibly to play a role in tissue- or cell type-specific gene expression by interacting with different homeodomain transcription factors. BANF1 compresses chromatin structure and interacts with the LEM domain of nuclear proteins to play a crucial role in membrane recruitment and chromatin decondensation during nuclear assembly. Additionally, retroviruses like HIV-1 incorporate BANF1 from host cells into preintegration complexes (PICs) to prevent autointegration of retroviral DNA and thereby promote integration of retroviral DNA into the host chromosome. |
| Application Dilution | |
| IF | 1:100-500 |
| Flow-Cyt | 1:100-500 |
Frequently asked questions
Which applications is BANF1 Recombinant Antibody, FITC Conjugated (bsm-54435R-FITC) validated for, and at what dilution?
bsm-54435R-FITC is validated for IF (1:100-500); Flow-Cyt (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BANF1 Recombinant Antibody, FITC Conjugated (bsm-54435R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54435R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BANF1 Recombinant Antibody, FITC Conjugated (bsm-54435R-FITC)?
Recombinant human BANF1
What are the excitation and emission wavelengths of the FITC conjugate (bsm-54435R-FITC)?
FITC on bsm-54435R-FITC: excitation / emission 494nm/518nm.