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ADORA1 Recombinant Antibody

The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ADORA1 antibody (bsm-54806R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ADORA1 antibody (bsm-54806R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-54806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-54806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-54806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-54806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt

Reactivity

  • Human
Overview
Catalog # bsm-54806r-100uL
Product Name ADORA1 Recombinant Antibody
Applications
IHC-P IHC-F IF FLOW-CYT
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human ADORA1
Clonality Recombinant
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 134
Swiss Prot P30542
Subcellular location Cell membrane
Synonyms RDC7; A1-AR; A1AR; A1R; AA1R; ARA1; Ri; AA1R_BOVIN; ADORA1; AA1R_HUMAN; AA1R_MOUSE; AA1R_RAT;
Application Dilution
IHC-P =1:100-200
IHC-F IHC-F=1:100-200
IF IF=1:50-200
Flow-Cyt Flow-Cyt=1:50-100

Frequently asked questions

Which applications is ADORA1 Recombinant Antibody (bsm-54806R) validated for, and at what dilution?

bsm-54806R is validated for IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IF (IF=1:50-200); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADORA1 Recombinant Antibody (bsm-54806R) react with?

Tested reactivity: Human.

How should bsm-54806R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADORA1 Recombinant Antibody (bsm-54806R)?

A synthesized peptide derived from human ADORA1

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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