GSDMD Recombinant Antibody

$THP-1 (H) differentiated with TPA (50 ng\/ml, overnight) and then LPS (5\u03bcg\/ml) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with GSDMD monoclonal antibody, unconjugated (bsm-55614R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) differentiated with TPA (50 ng\/ml, overnight) and then LPS (5\u03bcg\/ml) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with GSDMD monoclonal antibody, unconjugated (bsm-55614R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-55614r-100ul
Product Name	GSDMD Recombinant Antibody
Applications	WB IHC-F IF IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human GSDMD protein
Clonality	Recombinant
Clone #	5G9
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79792
Swiss Prot	P57764
Subcellular location	Cytoplasm, Secreted, Cell membrane, Extracellular matrix
Synonyms	DF5L; DFNA5L; FKSG10; GSDMDC1; GSDMD_HUMAN; GSDMD; Gasdermin domain-containing protein 1;
Application Dilution
WB
IHC-F
IF
IHC-P