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NCAM Monoclonal Antibody

4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NCAM) monoclonal Antibody, unconjugated (bsm-55630R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NCAM) monoclonal Antibody, unconjugated (bsm-55630R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NCAM) monoclonal Antibody, unconjugated (bsm-55630R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NCAM) monoclonal Antibody, unconjugated (bsm-55630R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-55630r-100uL
Product Name NCAM Monoclonal Antibody
Applications
ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NCAM
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P13591
Subcellular location Secreted, Cell membrane
Synonyms CD56; MSK39; NCAM; E-NCAM; NCAM-1; N-CAM; N-CAM-1; NCAM-C; NCAMC; NCAM1_HUMAN; NCAM1; NCAM1_MOUSE; NCAM1_RAT;
Application Dilution
ICC/IF =1:100-500

Frequently asked questions

Which applications is NCAM Monoclonal Antibody (bsm-55630R) validated for, and at what dilution?

bsm-55630R is validated for ICC/IF (ICC/IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NCAM Monoclonal Antibody (bsm-55630R) react with?

Tested reactivity: Human.

How should bsm-55630R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NCAM Monoclonal Antibody (bsm-55630R)?

KLH conjugated synthetic peptide derived from human NCAM

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