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NF-κB p65 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with NF-\u03baB p65 monoclonal antibody, unconjugated (bsm-55650R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with NF-\u03baB p65 monoclonal antibody, unconjugated (bsm-55650R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-\u03baB p65 Monoclonal Antibody, Unconjugated (bsm-55650R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NF-\u03baB p65 antibody (bsm-55650R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NF-\u03baB p65 antibody (bsm-55650R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55650r-100uL
Product Name NF-κB p65 Recombinant Antibody
Applications
WB IHC-F IF IHC-P FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NFKBp65
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5970
Swiss Prot Q04206
Subcellular location Cytoplasm, Nucleus
Synonyms AIF3BL3; CMCU; NFKB3; p65; p65 NF-kappa B; p65 NFkB; NFkB; nos2; TF65_HUMAN; RELA; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; TF65_MOUSE;
Application Dilution
WB =1:500-2000
IHC-F IHC-F=1:100-200
IF IF=1:100-200
IHC-P IHC-P=1:100-200
Flow-Cyt Flow-Cyt=1:50-100

Frequently asked questions

Which applications is NF-κB p65 Recombinant Antibody (bsm-55650R) validated for, and at what dilution?

bsm-55650R is validated for WB (WB=1:500-2000); IHC-F (IHC-F=1:100-200); IF (IF=1:100-200); IHC-P (IHC-P=1:100-200); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NF-κB p65 Recombinant Antibody (bsm-55650R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55650R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NF-κB p65 Recombinant Antibody (bsm-55650R)?

KLH conjugated synthetic peptide derived from human NFKBp65

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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