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Ubiquityl-Histone H2B (Lys120) Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bsm-60167r-100uL
Product Name Ubiquityl-Histone H2B (Lys120) Recombinant Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Ubiquityl-Histone H2B (Lys120)
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3018
Swiss Prot P33778
Subcellular location Nucleus
Synonyms Histone H2B/HIST1H2BD; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S; histone H2B type 1-B; H2B1B_HUMAN.
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008].
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:200-500
IHC-F 1:100-500
IF

Frequently asked questions

Which applications is Ubiquityl-Histone H2B (Lys120) Recombinant Antibody (bsm-60167R) validated for, and at what dilution?

bsm-60167R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:200-500); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Ubiquityl-Histone H2B (Lys120) Recombinant Antibody (bsm-60167R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bsm-60167R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Ubiquityl-Histone H2B (Lys120) Recombinant Antibody (bsm-60167R)?

KLH conjugated synthetic peptide derived from human Ubiquityl-Histone H2B (Lys120)

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