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CD47 Recombinant Antibody

The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD47 antibody (bsm-60739R,1:100):; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD47 antibody (bsm-60739R,1:100):; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD47) monoclonal Antibody, unconjugated (bsm-60739) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD47) monoclonal Antibody, unconjugated (bsm-60739) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • IF(ICC)
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-60739r-100uL
Product Name CD47 Recombinant Antibody
Applications
IF(ICC) FCM
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD47
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer PBS, Glycerol, BSA.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 961
Swiss Prot Q08722
Subcellular location Cell membrane
Synonyms Antigenic surface determinant protein OA3; CD 47; CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47_HUMAN; IAP; Integrin Associated Protein; Integrin associated signal transducer; Integrin-associated protein; Leukocyte surface antigen CD47; MER 6; MER6; OA 3; OA3; Protein MER6; Rh related antigen.
Application Dilution
IF(ICC) 1:50-200
FCM FCM1:50-100

Frequently asked questions

Which applications is CD47 Recombinant Antibody (bsm-60739R) validated for, and at what dilution?

bsm-60739R is validated for IF(ICC)(IF(ICC)) (1:50-200); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD47 Recombinant Antibody (bsm-60739R) react with?

Tested reactivity: Human.

How should bsm-60739R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS, Glycerol, BSA.

What immunogen was used to raise CD47 Recombinant Antibody (bsm-60739R)?

KLH conjugated synthetic peptide derived from human CD47

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