FOXP3 Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Antibody incubation with FOXP3 Monoclonal Antibody, Unconjugated(bsm-60760R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Antibody incubation with FOXP3 Monoclonal Antibody, Unconjugated(bsm-60760R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FOXP3 antibody (bsm-60760R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FOXP3 antibody (bsm-60760R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$25 ug total protein per lane of various lysates (see on figure) probed with FOXP3 monoclonal antibody, unconjugated (bsm-60760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with FOXP3 monoclonal antibody, unconjugated (bsm-60760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human

Overview
Catalog #	bsm-60760r
Product Name	FOXP3 Recombinant Antibody
Applications	IHC-P IHC-F IF(IHC-P)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FOXP3
Clonality	Recombinant
Clone #	5C7
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	PBS, Glycerol, BSA.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	50943
Swiss Prot	Q9BZS1
Subcellular location	Nucleus
Synonyms	AIID; DIETER; IPEX; JM2; PIDX; XPID; scurfin; sf; RGD1562112; FOXP3_HUMAN; FOXP3; FOXP3_MOUSE; D3ZKI1_RAT;
Application Dilution
IHC-P	1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500