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FOXP3 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Antibody incubation with FOXP3 Monoclonal Antibody, Unconjugated(bsm-60760R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Antibody incubation with FOXP3 Monoclonal Antibody, Unconjugated(bsm-60760R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FOXP3 antibody (bsm-60760R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FOXP3 antibody (bsm-60760R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with FOXP3 monoclonal antibody, unconjugated (bsm-60760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with FOXP3 monoclonal antibody, unconjugated (bsm-60760R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-60760R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-60760r-100uL
Product Name FOXP3 Recombinant Antibody
Applications
IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FOXP3
Clonality Recombinant
Clone # 5C7
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer PBS, Glycerol, BSA.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 50943
Swiss Prot Q9BZS1
Subcellular location Nucleus
Synonyms AIID; DIETER; IPEX; JM2; PIDX; XPID; scurfin; sf; RGD1562112; FOXP3_HUMAN; FOXP3; FOXP3_MOUSE; D3ZKI1_RAT;
Application Dilution
IHC-P 1:100-500
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is FOXP3 Recombinant Antibody (bsm-60760R) validated for, and at what dilution?

bsm-60760R is validated for IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FOXP3 Recombinant Antibody (bsm-60760R) react with?

Tested reactivity: Human.

How should bsm-60760R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS, Glycerol, BSA.

What immunogen was used to raise FOXP3 Recombinant Antibody (bsm-60760R)?

KLH conjugated synthetic peptide derived from human FOXP3

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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