PAP Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded (Human prostate tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PAP) Monoclonal Antibody, Unconjugated (bsm-60820R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human prostate tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PAP) Monoclonal Antibody, Unconjugated (bsm-60820R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human

Overview
Catalog #	bsm-60820r
Product Name	PAP Recombinant Antibody
Applications	IHC-P IHC-F IF(IHC-P)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55
Swiss Prot	P15309
Subcellular location	Secreted, Cell membrane
Synonyms	Acid phosphatase prostate; ACP 3; ACP3; ACPP; Ecto-5'-nucleotidase; Prostatic Acid Phosphatase ; PPAP_HUMAN; Prostatic acid phosphatase; Thiamine monophosphatase; TMPase; 5'-NT; 5'-nucleotidase.
Application Dilution
IHC-P	1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500