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IFI16 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IFI16 Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, bs-0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IFI16 Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, bs-0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF(IHC-P)
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-60844m-100uL
Product Name IFI16 Monoclonal Antibody
Applications
IHC-F IF(IHC-P) IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human IFI16
Clonality Monoclonal
Clone # 5C9
Isotype IgG1
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer PBS, Glycerol, BSA.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3428
Swiss Prot Q16666
Subcellular location Cytoplasm, Nucleus
Synonyms gamma-interferon-inducible protein Ifi16; Interferon-inducible myeloid differentiation transcriptional activator; A730048F03; D3; D3cDNA; Ifi-d3; IFI16; IFI16B; IFI204; IFI205; Ifi205a; Ifi205b; IFNGIP1; MGC9466; Mnda; p204; PYHIN2; IFI16; gamma interferon inducible protein 16; gamma-interferon-inducible protein Ifi16; IF16_HUMAN; interferon activated gene 204; Interferon gamma induced protein IFI 16; Interferon gamma inducible protein 16; Interferon-inducible myeloid differentiation transcriptional activator.
Application Dilution
IHC-F 1:50-200
IF(IHC-P) 1:50-200
IHC-P 1:50-200

Frequently asked questions

Which applications is IFI16 Monoclonal Antibody (bsm-60844M) validated for, and at what dilution?

bsm-60844M is validated for IHC-F (1:50-200); IF(IHC-P) (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IFI16 Monoclonal Antibody (bsm-60844M) react with?

Tested reactivity: Human.

How should bsm-60844M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS, Glycerol, BSA.

What immunogen was used to raise IFI16 Monoclonal Antibody (bsm-60844M)?

KLH conjugated synthetic peptide derived from human IFI16

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