L-Lactyl Lysine Recombinant Antibody

Name: L-Lactyl Lysine Recombinant Antibody
Brand: Bioss
SKU: bsm-60863R
Price: 490.0 USD
Availability: InStock

$4% Paraformaldehyde-fixed HeLa (treated with sodium lactate (100mM, 24hr)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (L-Lactyl Lysine) monoclonal Antibody, unconjugated (bsm-60863R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HeLa (treated with sodium lactate (100mM, 24hr)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (L-Lactyl Lysine) monoclonal Antibody, unconjugated (bsm-60863R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$The HeLa (treated with sodium lactate (100mM, 24hr)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-L-Lactyl Lysine antibody (bsm-60863R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The HeLa (treated with sodium lactate (100mM, 24hr)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-L-Lactyl Lysine antibody (bsm-60863R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with L-Lysine (lactyl) Monoclonal Antibody, Unconjugated (bsm-60863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with L-Lysine (lactyl) Monoclonal Antibody, Unconjugated (bsm-60863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with L-Lysine (lactyl) Monoclonal Antibody, Unconjugated (bsm-60863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with L-Lysine (lactyl) Monoclonal Antibody, Unconjugated (bsm-60863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IP
IF
ChIP
IHC-P
FCM
ICC/IF

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-60863r-100uL
Product Name	L-Lactyl Lysine Recombinant Antibody
Applications	WB IHC-F IP IF CHIP IHC-P FCM ICC/IF
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	L-Lactyl Lysine-KLH
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	PBS, Glycerol, BSA
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Background	Lactyl lysine is derivative of the amino acid residue lysine where the sidechain ammonium group undergoes lactylation.
Application Dilution
WB
IHC-F
IP
IF
ChIP
IHC-P
FCM
ICC/IF