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Phospho-RPS6 (Ser240 + Ser244) Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser240 + Ser244) monoclonal antibody, unconjugated (bsm-60943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser240 + Ser244) monoclonal antibody, unconjugated (bsm-60943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60943r-100uL
Product Name Phospho-RPS6 (Ser240 + Ser244) Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser240/244
Modification Site Ser240 + Ser244
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6194
Swiss Prot P62753
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms RPS6(phospho S240+S244); p-RPS6(phospho S240+S244); Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal(1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:50-200
IHC-F IHC-F1:50-200
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is Phospho-RPS6 (Ser240 + Ser244) Recombinant Antibody (bsm-60943R) validated for, and at what dilution?

bsm-60943R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-RPS6 (Ser240 + Ser244) Recombinant Antibody (bsm-60943R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-60943R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-RPS6 (Ser240 + Ser244) Recombinant Antibody (bsm-60943R)?

KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser240/244

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