Caspase-3 Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61071r-pe-100uL |
| Product Name | Caspase-3 Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Caspase-3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 836 |
| Swiss Prot | P42574 |
| Subcellular location | Cytoplasm |
| Synonyms | cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF(IHC-P) | IFIHC-P1:200-800 |
Frequently asked questions
Which applications is Caspase-3 Recombinant Antibody, PE Conjugated (bsm-61071R-PE) validated for, and at what dilution?
bsm-61071R-PE is validated for WB(WB) (1:1000-5000); IF(IHC-P)(IF(IHC-P)) (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-3 Recombinant Antibody, PE Conjugated (bsm-61071R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61071R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase-3 Recombinant Antibody, PE Conjugated (bsm-61071R-PE)?
KLH conjugated synthetic peptide derived from human Caspase-3
What are the excitation and emission wavelengths of the PE conjugate (bsm-61071R-PE)?
PE on bsm-61071R-PE: excitation / emission 496,564nm/578nm.