Phospho-PP2A alpha (Ser307) Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61107r-pe-100uL |
| Product Name | Phospho-PP2A alpha (Ser307) Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PP2A alpha around the phosphorylation site of S307 |
| Modification Site | Ser307 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5515 |
| Swiss Prot | P67775 |
| Subcellular location | Nucleus |
| Synonyms | eplication protein C; PP2A-alpha; Replication protein C; RP-C; PPP2CA; MGC786. |
| Background | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-PP2A alpha (Ser307) Recombinant Antibody, PE Conjugated (bsm-61107R-PE) validated for, and at what dilution?
bsm-61107R-PE is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PP2A alpha (Ser307) Recombinant Antibody, PE Conjugated (bsm-61107R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61107R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PP2A alpha (Ser307) Recombinant Antibody, PE Conjugated (bsm-61107R-PE)?
KLH conjugated synthetic peptide derived from human PP2A alpha around the phosphorylation site of S307
What are the excitation and emission wavelengths of the PE conjugate (bsm-61107R-PE)?
PE on bsm-61107R-PE: excitation / emission 496,564nm/578nm.