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Pygopus 2 Recombinant Antibody

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Pygopus 2 antibody (bsm-61113R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Pygopus 2 antibody (bsm-61113R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pygopus 2) monoclonal Antibody, unconjugated (bsm-61113R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Pygopus 2) monoclonal Antibody, unconjugated (bsm-61113R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61113r-20uL
Product Name Pygopus 2 Recombinant Antibody
Applications
FCM IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Pygopus 2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 90780
Swiss Prot Q9BRQ0
Subcellular location Nucleus
Synonyms PP7910; PYGO2; Pygo2 protein; Pygopus 2.
Background Involved in signal transduction through the Wnt pathway.
Application Dilution
FCM 1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is Pygopus 2 Recombinant Antibody (bsm-61113R) validated for, and at what dilution?

bsm-61113R is validated for FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Pygopus 2 Recombinant Antibody (bsm-61113R) react with?

Tested reactivity: Human, Mouse.

How should bsm-61113R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Pygopus 2 Recombinant Antibody (bsm-61113R)?

KLH conjugated synthetic peptide derived from human Pygopus 2

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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