Hsc70 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61188r-bf488-100uL |
| Product Name | Hsc70 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hsc70 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3312 |
| Swiss Prot | P11142 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Heat shock cognate 71 kDa protein; HS7C; HSP73; HSP7C; HSPA10; HSPA8. |
| Background | Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated autophagy, activation of proteolysis of misfolded proteins, formation and dissociation of protein complexes, and antigen presentation. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Hsc70 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61188R-BF488) validated for, and at what dilution?
bsm-61188R-BF488 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hsc70 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61188R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61188R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hsc70 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61188R-BF488)?
KLH conjugated synthetic peptide derived from human Hsc70
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61188R-BF488)?
AbBy Fluor-488 on bsm-61188R-BF488: excitation / emission 499nm/519nm.