PHB1 Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61208r-apc-100uL |
| Product Name | PHB1 Recombinant Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHB1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5245 |
| Swiss Prot | P35232 |
| Synonyms | PHB; PHB1; HEL 215; Epididymis secretory sperm binding protein Li 54e. |
| Background | PHB1 is 30kDa tumor suppressor protein involved in cell cycle control. PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation. In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PHB1 Recombinant Antibody, APC Conjugated (bsm-61208R-APC) validated for, and at what dilution?
bsm-61208R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHB1 Recombinant Antibody, APC Conjugated (bsm-61208R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61208R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PHB1 Recombinant Antibody, APC Conjugated (bsm-61208R-APC)?
KLH conjugated synthetic peptide derived from human PHB1
What are the excitation and emission wavelengths of the APC conjugate (bsm-61208R-APC)?
APC on bsm-61208R-APC: excitation / emission 650nm/660nm.