PRPF19 Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61268r-pe-100uL |
| Product Name | PRPF19 Recombinant Antibody, PE Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PRPF19 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27339 |
| Swiss Prot | Q9UMS4 |
| Synonyms | NMP200; nuclear matrix protein 200; pre-mRNA-processing factor 19; PRP19; PRP19/PSO4; PRPF19; PSO4; senescence evasion factor; SNEV; UBOX4. |
| Background | Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PRPF19 Recombinant Antibody, PE Conjugated (bsm-61268R-PE) validated for, and at what dilution?
bsm-61268R-PE is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRPF19 Recombinant Antibody, PE Conjugated (bsm-61268R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61268R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRPF19 Recombinant Antibody, PE Conjugated (bsm-61268R-PE)?
KLH conjugated synthetic peptide derived from human PRPF19
What are the excitation and emission wavelengths of the PE conjugate (bsm-61268R-PE)?
PE on bsm-61268R-PE: excitation / emission 496,564nm/578nm.