Caspase-10 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61328r-100uL |
| Product Name | Caspase-10 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IP
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Caspase 10 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 843 |
| Swiss Prot | Q92851 |
| Subcellular location | Cytoplasm |
| Synonyms | Apoptotic protease Mch4; CASP10; Caspase 10; FLICE2; ICE like apoptotic protease 4; FADD-like ICE2. |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IP | 1-2ug |
| IF | |
| Flow-Cyt | 1ug/Test |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is Caspase-10 Recombinant Antibody (bsm-61328R) validated for, and at what dilution?
bsm-61328R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IP (1-2ug); IF; Flow-Cyt (1ug/Test); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-10 Recombinant Antibody (bsm-61328R) react with?
Tested reactivity: Human, Mouse.
How should bsm-61328R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Caspase-10 Recombinant Antibody (bsm-61328R)?
KLH conjugated synthetic peptide derived from human Caspase 10