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Ubiquitin D Recombinant Antibody

Western blot analysis of HepG2 cell lysate. Using Ubiquitin D (bsm-61330R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Western blot analysis of HepG2 cell lysate. Using Ubiquitin D (bsm-61330R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquitin D Monoclonal Antibody, Unconjugated (bsm-61330R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61330r-20uL
Product Name Ubiquitin D Recombinant Antibody
Applications
WB IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FAT10
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10537
Swiss Prot O15205
Subcellular location Cytoplasm, Nucleus
Synonyms FAT10; UBD-3; GABBR1; UBD; Ubiquitin D.
Background Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2).
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF
ICC/IF 1:50-100

Frequently asked questions

Which applications is Ubiquitin D Recombinant Antibody (bsm-61330R) validated for, and at what dilution?

bsm-61330R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Ubiquitin D Recombinant Antibody (bsm-61330R) react with?

Tested reactivity: Human, Mouse.

How should bsm-61330R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Ubiquitin D Recombinant Antibody (bsm-61330R)?

KLH conjugated synthetic peptide derived from human FAT10

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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